Start here with the Bitesize Bio guide to measuring protein purity and quality. There’s not much I can help you with here because your workflow is intimately connected to your sample.īefore you jeer and call me a cop-out, let me point you to some unmissable resources! Cryo-EM: A Very Brief RecapĬryo-EM is electron microscopy performed on samples maintained at cryogenic temperatures ( 99%. Stick with me as I present 5 essential considerations for cryo-EM sample prep to maximize your results. Whether you’re about to embark on an ambitious project involving cryo-EM or just looking to expand your knowledge of the field, we’ve got you covered. But how can you ensure you get impressive and informative cryo-EM images? The key is good cryo-EM sample prep.Īll structures solved using cryo-EM started with an intact specimen in an aqueous solution.īut what makes a sample suitable for cryo-EM? What requirements does it impose upon them? And what do you rely on for quality control? Choose your sample grids carefully by researching grids used for similar molecules, and spend time optimizing your grid preparation.Ĭryo-EM offers a way to get near atomic-resolution structures of biological molecules, perfect for imaging proteins and DNA. CryoEM is expensive, so before imaging, optimize your cryoEM sample prep to ensure your sample is >99% pure, homogenous, and at a suitable concentration (between 10 nm and 10 µM).
0 kommentar(er)
